Inhibition ofin vitro mineralization by aluminum in a clonal osteoblastlike cell line, MC3T3-E1
- 1 September 1986
- journal article
- conference paper
- Published by Springer Nature in Calcified Tissue International
- Vol. 39 (5), 319-323
- https://doi.org/10.1007/bf02555198
Abstract
The direct effect of aluminum on mineralization was examined using an osteoblastlike cell line, MC3T3-E1. The mineralization process was quantitated by measuring45Ca accumulation into the cell and matrix layer of MC3T3-E1 cells in culture. The accumulation of45Ca into the cell and matrix layer increased dramatically after 13 days of culture without a parallel change in the DNA content of these cells. Because nodular clusters of cells appear around the same period in which a massive mineralization occurs, the marked increase in45Ca accumulation after the 13th day of culture appears to represent deposition of45Ca into the extracellular matrix. Thus, this culture system offers a useful model for making a quantitative estimation of osteoblast-mediated mineralizationin vitro. When aluminum was added to this system, the accumulation of45Ca into the cell matrix layer was inhibited in a dose-dependent manner: 10−6 M aluminum reduced45Ca accumulation to 40.8±2.7% of that in nontreated cells without affecting alkaline phosphatase activity or the DNA content of these cells. Because the concentration of aluminum used in this study is well within the range of serum aluminum levels seen in chronic dialysis patients, the direct effects of aluminum on osteoblast-mediated mineralization shown in the present study may underlie the development of so-called aluminum-induced “osteomalacia” in certain dialysis patients.This publication has 23 references indexed in Scilit:
- Aluminum deposition at the osteoid-bone interface. An epiphenomenon of the osteomalacic state in vitamin D-deficient dogs.JCI Insight, 1985
- Effects of parathyroid hormone on cAMP production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cellsBiochemical and Biophysical Research Communications, 1984
- The Use of Deferoxamine in the Management of Aluminum Accumulation in Bone in Patients with Renal FailureNew England Journal of Medicine, 1984
- Short-term aluminum administration in the rat. Effects on bone formation and relationship to renal osteomalacia.JCI Insight, 1984
- Suppression of parathyroid hormone secretion by aluminumKidney International, 1983
- Parathyroid gland responsiveness to acute hypocalcemia in dialysis osteomalaciaKidney International, 1983
- In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria.The Journal of cell biology, 1983
- The Prevalence of Bone Aluminum Deposition in Renal Osteodystrophy and Its Relation to the Response to Calcitriol TherapyNew England Journal of Medicine, 1982
- Aluminum localization in bone from hemodialyzed patients: Relationship to matrix mineralizationKidney International, 1981
- Bone aluminium in haemodialysed patients and in rats injected with aluminium chloride: relationship to impaired bone mineralisation.Journal of Clinical Pathology, 1979