Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.
- 1 December 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (23), 7132-7136
- https://doi.org/10.1073/pnas.79.23.7132
Abstract
Sequences located upstream from the T-A-T-A box, between positions -97 and -34, are necessary for efficient in vivo transcription from the adenovirus serotype 2 major late promoter. The effect of these upstream sequences was also investigated in vitro using a whole cell or an S100 extract and circular or linear templates. With the whole cell extract, the in vivo effect of the upstream sequences was reproduced in vitro. With the S100 extract, some effect of the upstream sequences was observed with circular, but not with linear, templates. [Human cervical carcinoma HeLa cells were used in this study.].This publication has 33 references indexed in Scilit:
- DNA sequences necessary for transcription of the rabbit β-globin gene in vivoNature, 1982
- Expression of a β-globin gene is enhanced by remote SV40 DNA sequencesCell, 1981
- SV40 gene expression is modulated by the cooperative binding of T antigen to DNACell, 1981
- Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: Identification of an upstream control regionCell, 1981
- Localization of DNA sequences necessary for transcription of the rabbit β-globin gene in vitroCell, 1981
- Organization and Expression of Eucaryotic Split Genes Coding for ProteinsAnnual Review of Biochemistry, 1981
- The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinantsNucleic Acids Research, 1981
- Promoter Sequences of Eukaryotic Protein-Coding GenesScience, 1980
- Sequence analysis of adenovirus DNAJournal of Molecular Biology, 1979
- Folding of the DNA double helix in chromatin-like structures from simian virus 40.Proceedings of the National Academy of Sciences, 1975