Immunosuppression by Moloney Leukemia Virus: Lack of Correlation Between Virus Replication and the Immunosuppressive Effect2
- 1 April 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 56 (4), 819-822
- https://doi.org/10.1093/jnci/56.4.819
Abstract
Young adult mice were infected with 104 plaque-forming units (PFU) of Moloney murine leukemia virus M-MuLV. Two different virus preparations were used: a) M-MuLV obtained from serial passage in mice [animal passage (AP)] and b) tissue culture (TC)-grown virus harvested after three in vitro passages of the AP M-MuLV in fibroblasts. Replication of TC and AP M-MuLV in spleen cells was determined by an infectious center (IC) assay at 1 and 2 weeks after the infection. Immune responsiveness of spleen cells was evaluated in challenge with sheep red blood cells (SRBC) and subsequent enumeration of antibody plaque-forming cells (PFC). TC M-MuLV replicated faster in the spleen than did AP M-MuLV and reached about 10- to 100-fold higher titers. However, the response of anti-SRBC PFC, suppressed to the same degree in the spleens of mice infected with TC or AP virus, was from 10 to 50% of the control response. A comparison of virus replication with the anti-SRBC response in aliquots from the same spleens showed no correlation between virus IC and antibody PFC. Both TC and AP M-MuLV induced the expression of virus-specific, cell membrane antigen on spleen cells. These findings indicated a divergence between virus replication on the one hand and the immunosuppressive effect and the cell membrane alteration on the other.Keywords
This publication has 3 references indexed in Scilit:
- Antibody Formation, Latency and Leukemia: Infection with Moloney VirusThe Journal of Immunology, 1966
- Antigenic Properties of Lymphomas Induced by the Moloney Agent2JNCI Journal of the National Cancer Institute, 1964
- Plaque Formation in Agar by Single Antibody-Producing CellsScience, 1963