Regulation of vascular endothelial growth factor expression by acidosis in human cancer cells
- 21 June 2001
- journal article
- research article
- Published by Springer Nature in Oncogene
- Vol. 20 (28), 3751-3756
- https://doi.org/10.1038/sj.onc.1204500
Abstract
The influence of acidosis on the expression of the vascular endothelial growth factor (VEGF) gene was determined. FG human pancreatic adenocarcinoma cells were incubated for various time periods in media at a physiologically relevant pH level (6.7–7.4). The expression of VEGF mRNA and protein secretion was inversely correlated with pH in a pH- and time-dependent manner. Transient acidosis also activated the VEGF promoter/enhancer luciferase reporter, which was consistent with an increased VEGF gene transcription rate and VEGF mRNA half-life. These data indicated that acidosis transcriptionally and posttranscriptionally regulates VEGF expression, suggesting that an acidic tumor microenvironment contributes to tumor angiogenesis and progression.Keywords
This publication has 30 references indexed in Scilit:
- Acidosis Inhibits Endothelial Cell Apoptosis and Function and Induces Basic Fibroblast Growth Factor and Vascular Endothelial Growth Factor ExpressionCirculation Research, 2000
- Regulation of vascular endothelial growth factor (VEGF) expression is mediated by internal initiation of translation and alternative initiation of transcriptionOncogene, 1998
- Low Environmental pH Is Responsible for the Induction of Nitric-oxide Synthase in MacrophagesJournal of Biological Chemistry, 1998
- Identification of a Human VPF/VEGF 3′ Untranslated Region Mediating Hypoxia-induced mRNA StabilityMolecular Biology of the Cell, 1998
- Modulation of VEGF production by pH and glucose in retinal Müller cellsCurrent Eye Research, 1998
- The molecular basis of the hypoxia response pathway: tumour hypoxia as a therapy target.Cancer and Metastasis Reviews, 1998
- A novel bHLH-PAS factor with close sequence similarity to hypoxia-inducible factor 1α regulates the VEGF expression and is potentially involved in lung and vascular developmentProceedings of the National Academy of Sciences, 1997
- HRF, a putative basic helix-loop-helix-PAS-domain transcription factor is closely related to hypoxia-inducible factor-1α and developmentally expressed in blood vesselsMechanisms of Development, 1997
- Tumor angiogenesis and tissue factorNature Medicine, 1996
- Hypoxia-Induced Transcription of the Vascular Endothelial Growth Factor Gene Is Independent of Functional Ap-1 Transcription FactorBiochemical and Biophysical Research Communications, 1995