Cryoprotective effects of various saccharides on cryopreserved mouse sperm from various strains
Open Access
- 7 November 2007
- journal article
- Published by Wiley in Reproductive Medicine and Biology
- Vol. 6 (4), 229-233
- https://doi.org/10.1111/j.1447-0578.2007.00190.x
Abstract
Cryopreservation of mouse sperm commonly uses raffinose, which is a trisaccharide, plus 3% skim milk. Because of the present lack of knowledge of the effectiveness of any other saccharides, we examined the cryoprotective effects of various saccharides on the viability of mouse sperm from various strains to determine which saccharides are the best cryoprotectants for mouse sperm. Sperm from the caudae epididymides of mature C57BL/6J mice were frozen with monosaccharides (fructose, glucose, rhamnose, xylose), disaccharides (lactose, maltose, sucrose, trehalose) or trisaccharides (melezitose, raffinose) in a range of concentrations (4–33%). After thawing, the optimal concentration was determined to be the concentration in which there was the highest proportion of motile sperm. In addition, sperm of inbred and hybrid mice were frozen with the saccharides at the optimal concentrations and used for in vitro fertilization. The optimal concentration was 12% for the disaccharides and 18% for the trisaccharides. The fertility of all strains, except C57BL/6J, showed the best cryoprotective effects with maltose, melezitose and raffinose when compared with fresh sperm. Maltose, melezitose and raffinose have the best effects when used as a protectant for cryopreservation of mouse sperm.Keywords
This publication has 32 references indexed in Scilit:
- Decrease of Fertilizing Ability of Mouse Spermatozoa after Freezing and Thawing Is Related to Cellular Injury1Biology of Reproduction, 2004
- Positive Effect of Partial Zona-Pellucida Dissection on the in Vitro Fertilizing Capacity of Cryopreserved C57BL/6J Transgenic Mouse Spermatozoa of Low MotilityBiology of Reproduction, 1997
- Normal Mice Develop From Oocytes Injected With Spermatozoa with Grossly Misshapen Heads1Biology of Reproduction, 1996
- Solute Accumulation and Compartmentation during the Cold Acclimation of Puma RyePlant Physiology, 1992
- Fertilizing capacity of frozen boar semen following surgical inseminationPublished by Wiley ,1970
- COMPLETE DEVELOPMENT IN VITRO OF THE PRE-IMPLANTATION STAGES OF THE MOUSE IN A SIMPLE CHEMICALLY DEFINED MEDIUMReproduction, 1968
- THE FERTILITY OP FKOZEN RAM AND BULL SEMENAustralian Veterinary Journal, 1955
- Fertilizing Capacity of Frozen Human SpermatozoaNature, 1953
- Fertilizing Capacity of Bull Spermatozoa after Freezing at −79° C.Nature, 1952
- THE LOW TEMPERATURE STORAGE OF RAM, BULL AND RABBIT SPERMATOZOAAustralian Veterinary Journal, 1950