Freely Suspended Cellular “Backpacks” Lead to Cell Aggregate Self-Assembly
Open Access
- 8 June 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 11 (7), 1826-1832
- https://doi.org/10.1021/bm100305h
Abstract
Cellular “backpacks” are a new type of anisotropic, nanoscale thickness microparticle that may be attached to the surface of living cells creating a “bio-hybrid” material. Previous work has shown that these backpacks do not impair cell viability or native functions such as migration in a B and T cell line, respectively. In the current work, we show that backpacks, when added to a cell suspension, assemble cells into aggregates of reproducible size. We investigate the efficiency of backpack−cell binding using flow cytometry and laser diffraction, examine the influence of backpack diameter on aggregate size, and show that even when cell−backpack complexes are forced through small pores, backpacks are not removed from the surfaces of cells.Keywords
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