Sodium and gating current time shifts resulting from changes in initial conditions.
Open Access
- 1 June 1983
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 81 (6), 773-784
- https://doi.org/10.1085/jgp.81.6.773
Abstract
The Na and gating currents of the squid [Loligo pealei] giant axon elicited by a depolarizing pulse are delayed, with little change in shape, as a result of a hyperpolarizing prepulse. The delays are almost completely saturated, at .apprx. 45 .mu.s, for prepulses to -140 mV. At 8.degree. C they develop with time constants of between 60-180 .mu.g for prepulses in the -130- to -50-mV range. There is a correlation between the extra charge moved during the gating current and the increase in the time delay of the Na current as the magnitude of the hyperpolarizing prepulse is increased. These results strengthen the conclusion that the gating current is indeed closely associated with the process of Na channel opening and provide information concerning the kinetics of the early steps, which are hidden in ionic current measurements. The main features of the gating and Na current time shifts and the correlation between charge movement and time shifts are duplicated by a sequential 6-state model for Na activation.Keywords
This publication has 10 references indexed in Scilit:
- Distribution and kinetics of membrane dielectric polarization. 1. Long-term inactivation of gating currents.The Journal of general physiology, 1982
- Distribution and kinetics of membrane dielectric polarization. II. Frequency domain studies of gating currents.The Journal of general physiology, 1982
- Membrane charge moved at contraction thresholds in skeletal muscle fibresThe Journal of Physiology, 1981
- Fast and slow steps in the activation of sodium channels.The Journal of general physiology, 1979
- Initial conditions and the kinetics of the sodium conductance in Myxicola giant axons. I. effects on the time-course of the sodium conductance.The Journal of general physiology, 1978
- Inactivation of the sodium channel. II. Gating current experiments.The Journal of general physiology, 1977
- The temporal and steady‐state relationships between activation of the sodium conductance and movement of the gating particles in the squid giant axon.The Journal of Physiology, 1976
- Charge Movement Associated with the Opening and Closing of the Activation Gates of the Na ChannelsThe Journal of general physiology, 1974
- Analysis of Certain Errors in Squid Axon Voltage Clamp MeasurementsBiophysical Journal, 1960
- A quantitative description of membrane current and its application to conduction and excitation in nerveThe Journal of Physiology, 1952