Rye cytology, cytogenetics and genetics — current status

Abstract

Progress in rye karyology is reviewed with respect to chromosome structure, recognition and chromosome nomenclature. Considerable contributions have been brought about by molecular techniques which have even revealed nucleotide sequences of some of the ribosomal DNA. DNA sequence organization correlates with the distribution of major Giemsa C-band regions as well as with N-bands and the binding sites of fluorescent dyes. The several banding patterns permit the classification of rye chromosomes. The increased data and widespread application of banding analysis require a consistent system of chromosome and/or band designation. Therefore, a standard band nomenclature is proposed with reference to the recommendations of the “Paris Conference on Standardization in Human Cytogenetics”. In addition, advances in genetics are summarized and discussed. Based on the original accepted standard karyogram and banding patterns of the rye chromosomes, meanwhile, 120 genes determining several characters have been associated with individual chromosomes and/or chromosome arms, including linkage studies for about 19 arrangements. Most results were obtained using wheat-rye addition lines as well as test crosses with defined translocations. Moreover, genetical studies based on appropriate trisomic and telotrisomic material resulted in the localization of 19 genes, including their linkage relationships.