Cardiolipin: a stereospecifically spin-labeled analogue and its specific enzymic hydrolysis.
- 1 March 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (3), 1227-1231
- https://doi.org/10.1073/pnas.75.3.1227
Abstract
The spin-labeled bovine cardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-(16-doxylstearoyl)glycero(3)phospho]-sn-glycerol was prepared. The stereoselective synthesis makes use of the monolysocardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-lyso-sn-glycero(3)phospho]-sn-glycerol, available from the stereospecific hydrolysis of cardiolipin by phospholipase A2 (phosphatide 2-acylhydrolase, EC 3.1.1.4) of Trimeresurus flavoviridis venom. The results of treatment of the spin-labeled cardiolipin with the cardiolipin-specific phospholipase D (phosphatidylcholine phosphatidohydrolase, EC 3.1.4.4) (Hemophilus (Haemophilus) parainfluenzae) of known specificity and with phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) of Bacillus cereus are consistent with the assigned structure. The spin-labeled cardiolipin is further characterized and the unique features of this diastereomer are discussed in the context of the unusual stereochemistry of the natural phospholipid.This publication has 21 references indexed in Scilit:
- Phosphatidylcholine exchange between the boundary lipid and bilayer domains in cytochrome oxidase containing membranesBiochemistry, 1977
- Sendai virus-induced hemolysis: reduction in heterogeneity of erythrocyte lipid bilayer fluidity.Proceedings of the National Academy of Sciences, 1977
- Selective enzymatic radioactive and spin labelling of phospholipids in biological membranes: application to study of temperature-induced changes of microsomal phosphatidylinositols and mitochondrial polyglycerophosphatidesCanadian Journal of Biochemistry, 1977
- Studies on Hydrolysis of Cardiolipin by Snake Venom Phospholipase AThe Journal of Biochemistry, 1965
- On the Structure of Cardiolipin*Biochemistry, 1964
- The stereochemical configuration of phosphatidyl glycerolBiochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects, 1964
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959
- Phosphatides of Pig Heart Cell FractionsJournal of Biological Chemistry, 1958
- The Phosphatide Composition of a Purified Cytochrome Oxidase PreparationJournal of Biological Chemistry, 1958
- A NOTE ON THE ASYMMETRICAL METABOLISM OF GLYCEROLJournal of Biological Chemistry, 1954