Expression of tumor‐associated protein variants in chemically induced rat hepatomas and transformed rat liver cell lines determined by two‐dimensional electrophoresis
- 1 January 1994
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 15 (1), 372-381
- https://doi.org/10.1002/elps.1150150156
Abstract
High resolution two‐dimensional electrophoresis (2‐DE), using the rat liver as a model, was applied to study hepatocarcinogenesis induced by different chemical carcinogens. Several tumor‐associated protein variants were detected by 2‐DE in chemically induced rat hepatomas and transformed rat liver cell lines compared to normal rat liver tissue. Proliferation‐related protein changes and/or protein alterations due to culture conditions were corrected for by comparison with 2‐DE patterns of isolated cells from regenerating liver and short‐term cultivated liver cells. Some of the tumor‐associated variants were further characterized: (i) By peptide mapping with limited proteolysis we detected clear relationships between several variants. (ii) By studying posttranslational modifications phosphorylated and glycosylated variants could be determined. (iii) A tumor‐associated protein variant was identified by amino acid analysis and amino acid sequencing.Keywords
This publication has 74 references indexed in Scilit:
- Identification of tissue proteins by amino acid analysis after purification by two-dimensional electrophoresisProtein Journal, 1992
- Two-dimensional electrophoretic analysis of transformation-sensitive polypeptides during chemically, spontaneously, and oncogene-induced transformation of rat liver epithelial cellsElectrophoresis, 1992
- Localization, isolation and properties of three NADPH-dependent aldehyde reducing enzymes from dog kidneyBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991
- Purification and characterization of the recombinant human aldose reductase expressed in baculovirus systemBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991
- Characterization of mRNA and genes for aldose reductase in ratBiochemical and Biophysical Research Communications, 1988
- Aldose reductase and ϱ‐crystallin belong to the same protein superfamily as aldehyde reductaseFEBS Letters, 1987
- Aldose metabolism in developing human fetal brain and liverCellular and Molecular Life Sciences, 1984
- The distribution of polyol:NADP oxidoreductase in mammalian tissuesBiochemical and Biophysical Research Communications, 1969
- Le mécanisme de la formation du fructose séminal et du fructose foetalBiochimica et Biophysica Acta, 1959
- L'aldose-réductaseBiochimica et Biophysica Acta, 1959