Kv2.1 and electrically silent Kv6.1 potassium channel subunits combine and express a novel current
- 2 December 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 399 (1-2), 177-182
- https://doi.org/10.1016/s0014-5793(96)01316-6
Abstract
Heteromultimer formation between Kv potassium channel subfamilies with the production of a novel current is reported for the first time. Protein-protein interactions between Kv2.1 and electrically silent Kv6.1 α-subunits were detected using two microelectrode voltage clamp and yeast two-hybrid measurements. Amino terminal portions of Kv6.1 were unable to form homomultimers but interacted specifically with amino termini of Kv2.1. Xenopus oocytes co-injected with Kv6.1 and Kv2.1 cRNAs exhibited a novel current with decreased rates of deactivation, decreased sensitivity to TEA block, and a hyperpolarizing shift of the half maximal activation potential when compared to Kv2.1. Our results indicate that Kv channel subfamilies can form heteromultimeric channels and, for the first time, suggest a possible functional role for the Kv6 subfamily.Keywords
This publication has 27 references indexed in Scilit:
- Kir2.2v: a possible negative regulator of the inwardly rectifying K+ channel Kir2.2FEBS Letters, 1996
- Molecular Cloning and Expression of a Modulatory Subunit of the Cyclic Nucleotide-gated Cation ChannelPublished by Elsevier ,1996
- Assembly of Voltage-gated Potassium ChannelsPublished by Elsevier ,1995
- Molecular recognition and assembly sequences involved in the subfamily-specific assembly of voltage-gated K+ channel subunit proteinsNeuron, 1995
- A second subunit of the olfactory cyclic nucleotide-gated channel confers high sensitivity to cAMPNeuron, 1994
- A new subunit of the cyclic nucleotide-gated cation channel in retinal rodsNature, 1993
- Shaker, Shal, Shab, and Shaw express independent K+ current systemsNeuron, 1991
- Voltage-sensing residues in the S4 region of a mammalian K+ channelNature, 1991
- Mutations Affecting Tea Blockade and Ion Permeation in Voltage-activated K + ChannelsScience, 1990
- Heteropolymeric potassium channels expressed in xenopus oocytes from cloned subunitsNeuron, 1990