Defibrination of Normal Human Blood in Vitro: a Method giving a High Recovery of Untraumatized Cells

Abstract

A new automatic apparatus is described for the defibrination of blood in vitro at laboratory temperature which results in only small (< 10%) losses of white cells. Freshly-drawn blood is mixed smoothly at high speed (> 1200 rpm) and fibrin is removed rapidly as formed. The apparatus is designed to produce minimum mechanical trauma to the blood. Subsequent in vitro incubation of red cells, granulocytes and lymphocytes demonstrated their viability to be comparable with cells obtained by other methods of defibrination.