Alkaloids and Plant Metabolism. VII. The Kinetin-Produced Elevation in Tyramine Methylpherase Levels

Abstract
Feeding kinetin to barley embryos in culture increased the activity of tyramine methylpherase (S-adeno-sylmethionine: tyramine methyltransferase) in the roots. This action of kinetin was prevented by simultaneous feeding of various inhibitors of protein synthesis. Kinetin did not affect the levels of 4 other enzymes present in the roots. Several kinetin analogs also stimulated tyramine methylpherase synthesis, but no other type of hormone or nutrient tested could duplicate the kinetin effect. The data indicate that kinetin stimulates de novo synthesis of tyramine methylpherase, rather than slowing its inactivation.