Crossover and noncrossover recombination during meiosis: timing and pathway relationships.
- 29 August 1995
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 92 (18), 8512-8516
- https://doi.org/10.1073/pnas.92.18.8512
Abstract
During meiosis, crossovers occur at a high level, but the level of noncrossover recombinants is even higher. The biological rationale for the existence of the latter events is not known. It has been suggested that a noncrossover-specific pathway exists specifically to mediate chromosome pairing. Using a physical assay that monitors both crossovers and noncrossovers in cultures of yeast undergoing synchronous meiosis, we find that both types of products appear at essentially the same time, after chromosomes are fully synapsed at pachytene. We have also analyzed a situation in which commitment to meiotic recombination and formation of the synaptonemal complex are coordinately suppressed (mer1 versus mer1 MER2++). We find that suppression is due primarily to restoration of meiosis-specific double-strand breaks, a characteristic of the major meiotic recombination pathway. Taken together, the observations presented suggest that there probably is no noncrossover-specific pathway and that restoration of intermediate events in a single pairing/recombination pathway promotes synaptonemal complex formation. The biological significant of noncrossover recombination remains to be determined, however.Keywords
This publication has 26 references indexed in Scilit:
- A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiaeCell, 1990
- MER1, a yeast gene required for chromosome pairing and genetic recombination, is induced in meiosis.Molecular and Cellular Biology, 1990
- Analysis of wild-type and rad50 mutants of yeast suggests an intimate relationship between meiotic chromosome synapsis and recombinationCell, 1990
- Double-strand breaks at an initiation site for meiotic gene conversionNature, 1989
- A Method for Gene Disruption That Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast StrainsGenetics, 1987
- Gene conversion, recombination nodules, and the initiation of meiotic synapsisBioEssays, 1987
- Gene conversions and their relation to homologous chromosome pairingPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1986
- Genetics: Gene conversion in the absence of reciprocal recombinationNature, 1984
- Physical Monitoring of Meiotic Recombination in Saccharomyces cerevisiaeCold Spring Harbor Symposia on Quantitative Biology, 1984
- Synaptonemal complex and crossing-over: structural support or interference?Heredity, 1978