N2-Guanine specific transfer RNA methyltransferase II from rat liver

Abstract
N2-guanine methyltransferase II was purified from rat liver. This enzyme methylated bulk E. coli tRNA to an extent of 7.6 nmoles of methyl groups/mg tRNA. Oligonucleotide analysis showed that N2-methylated guanosines were present in the modified tRNA in two sequences, namely Y-m2G-Cp and Y-m22G-Cp in the ratio 4:3.