Effect of Loss of Thymidine Kinase Activity on the Tumorigenicity of Clones of SV40-Transformed Hamster Cells
- 1 October 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (2), 1042-1049
- https://doi.org/10.1073/pnas.67.2.1042
Abstract
Cells deficient in the enzyme thymidine kinase were derived from transplantable SV40-transformed hamster cells. The resultant cell lines were less transplantable when inoculated into hamsters. Tumors which did arise from such cells had prolonged latent periods and were found to contain a mixture of enzyme-containing and enzyme-deficient cells. Revertant cell lines obtained either spontaneously or after mutagenesis in vitro contained intermediate levels of thymidine kinase activity and displayed an oncogenic potential which was intermediate between the wild type and enzyme-deficient cells. It is postulated that salvage pathway enzymes may play a rate-limiting role in tumorigenesis.Keywords
This publication has 24 references indexed in Scilit:
- Exchange between hamster cells in cultureExperimental Cell Research, 1968
- Comparative study of the thymidine kinase and thymidylate kinase activities and of the feedbach inhibition of thymidine kinase in normal and neoplastic human tissue.1968
- Ultraviolet Irradiation of Mouse L Cells: Effects on DNA Synthesis and Progression through the Cell CycleRadiation Research, 1968
- The Control of Nucleotide BiosynthesisAnnual Review of Biochemistry, 1968
- An Analysis of SV40-Induced Transformation of Hamster Kidney Tissue In Vitro. IV. Studies of the Pathology of Hamster Tumors Induced With SV40-Transformed Hamster Cell ClonesJNCI Journal of the National Cancer Institute, 1966
- An Analysis of SV40-Induced Transformation of Hamster Kidney Tissue In Vitro. III. Persistence of SV40 Viral Genome in Clones of Transformed Hamster Cells2JNCI Journal of the National Cancer Institute, 1966
- Physiological Thymidine Reutilization in Rat Bone Marrow.Experimental Biology and Medicine, 1966
- Studies on Thymidine Kinase of Human Liver Cells in CultureJournal of Biological Chemistry, 1966
- Tissue cultures and mycoplasmas.1965
- Inhibiyors of protein synthesis: Effect on the levels of deoxycytidylate deaminase, thymidylate synthetase, and thymidine kinase in regenerating rat liverBiochemical and Biophysical Research Communications, 1965