Quantitation of Serotonin in Human Plasma, Serum and Cerebrospinal Fluid Samples by HPLC-EC Using 6-Hydroxytryptamine as an Internal Standard
- 1 November 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (13), 2679-2689
- https://doi.org/10.1080/01483918408067035
Abstract
A simple method for sample clean up and concentration of serotonin (5-HT) in biological samples, such as human cerebro-spinal fluid and serum, is described. To the sample 6-hydroxy-tryptamine (6-HT) is added as an internal standard and it is then absorbed either on C18 SEP-PAK cartridge or Biorex-70 short column. 5-HT and 6-HT are then eluted from the column with methanolic formic acid. After evaporation, the residue is dissolved in the mobile phase and an aliquot is used for LC-EC quantitation.This publication has 8 references indexed in Scilit:
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