Coding sequence and flanking regions of the mouse vimentin gene

Abstract

Using a polyclonal antibody, a cDNA clone coding for part of mouse vimentin was identified in a λgt11 expression library. DNA from this clone was used to screen a genomic library from Ehrlich Ascites Tumor cells for the mouse vimentin gene. A clone was found which contained the whole coding sequence and a large part of the 5′- and 3′-untranslated sequences. It was used to prepare a construct equivalent to a full-length cDNA clone. Extensive homologies to the vimentin sequence from other species were found for the coding and 3′-untranslated sequences and the promoter region.