Selective Expression of DM‐20, an Alternatively Spliced Myelin Proteolipid Protein Gene Product, in Developing Nervous System and in Nonglial Cells
- 1 June 1992
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 58 (6), 2248-2253
- https://doi.org/10.1111/j.1471-4159.1992.tb10970.x
Abstract
Mutations within the gene for myelin proteolipid protein (PLP), a major myelin structural protein, result in abnormal glial differentiation, suggesting that the PLP gene products play some other functional roles. Transcripts from the PLP gene were analyzed in the developing mouse brain by a sensitive method using polymerase chain reaction. The mRNA for DM-20, an alternatively spliced transcript from the PLP gene, was detected in the embryonic mouse brain as early as embryonic day 11, long before the appearance of oligodendrocytes, which were considered to be responsible for PLP production. PLP gene expression was analyzed in various cell lines to determine whether synthesis of the DM-20 mRNA is restricted to those of glial cell lineage. All of the nervous system cell lines examined, including nonglial cell lines, produced DM-20 mRNA but no or very little PLP mRNA. Peripheral sciatic nerve from adult Wistar rats also produced mainly DM-20 mRNA. These results indicate that DM-20 is not only a myelin structural protein, but it also plays other roles in the nervous system that seem to relate, at least in part, to glial differentiation.Keywords
This publication has 24 references indexed in Scilit:
- Proteolipid DM-20 predominates over PLP in peripheral nervous systemNeuroReport, 1991
- Developmental Study of Proteolipids in Bovine Brain: A Novel Proteolipid and DM‐20 Appear Before Proteolipid Protein (PLP) During MyelinationJournal of Neurochemistry, 1990
- Expression of myelin protein genes in Schwann cellsJournal of Neurocytology, 1989
- Myelin proteolipid protein gene structure and its regulation of expression in normal and jimpy mutant miceJournal of Molecular Biology, 1988
- Glial conditioned medium enables jimpy oligodendrocytes to express properties of normal oligodendrocytes: Production of myelin antigens and membranesGlia, 1988
- A Single Nucleotide Difference in the Gene for Myelin Proteolipid Protein Defines the Jimpy Mutation in MouseJournal of Neurochemistry, 1987
- An AG→GG transition at a splice site in the myelin proteolipid protein gene in jimpy mice results in the removal of an exonFEBS Letters, 1987
- Myelin‐specific proteolipid protein is expressed in myelinating schwann cells but is not incorporated into myelin sheathsJournal of Neuroscience Research, 1987
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984
- Myelin deficit in the Jimpy mouse may be due to cellular abnormalities in astrogliaNature, 1976