Transfer of a Mycobacterium tuberculosis Genotyping Method, Spoligotyping, from a Reverse Line-Blot Hybridization, Membrane-Based Assay to the Luminex Multianalyte Profiling System
Open Access
- 1 January 2004
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (1), 474-477
- https://doi.org/10.1128/jcm.42.1.474-477.2004
Abstract
Spoligotyping using Luminex technology was shown to be a highly reproducible method suitable for high-throughput analysis. Spoligotyping of 48 isolates using the traditional membrane-based assay and the Luminex assay yielded concordant results for all isolates. The Luminex platform provides greater flexibility and cost effectiveness than the membrane-based assay.Keywords
This publication has 4 references indexed in Scilit:
- Improvement of Differentiation and Interpretability of Spoligotyping for Mycobacterium tuberculosis Complex Isolates by Introduction of New Spacer OligonucleotidesJournal of Clinical Microbiology, 2002
- Global Distribution of Mycobacterium tuberculosis SpoligotypesEmerging Infectious Diseases, 2002
- Genetic Variation and Evolutionary Origin of the Direct Repeat Locus of Mycobacterium tuberculosis Complex BacteriaJournal of Bacteriology, 2000
- Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by SpoligotypingPublished by Springer Nature ,1997