Transcription of the Vibrio cholerae haemolysin gene, hlyA, and cloning of a positive regulatory locus, hlyU
- 1 August 1991
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 5 (8), 2031-2038
- https://doi.org/10.1111/j.1365-2958.1991.tb00825.x
Abstract
Transcription of the Vibrio cholerae hlyA gene, which encodes a cytotoxic haemolysin, has been investigated. The hlyA transcript initiates 430 nucleotides (nt) upstream of the translational start site. hlyA-cat transcriptional fusion constructs were active in V. cholerae but not in Escherichia coli. An hlyA-cat fusion was used to select, from a V. cholerae O17 plasmid library, a clone that could activate the hlyA promoter in E. coli. This regulatory locus has been designated hlyU. hlyU appears to be distinct from the previously described hlyR locus.Keywords
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