Histochemical localisation of monoamine oxidase A and B in rat brain
- 1 February 1988
- journal article
- research article
- Published by Springer Nature in Journal of Neural Transmission
- Vol. 74 (1), 29-42
- https://doi.org/10.1007/bf01243573
Abstract
The histochemical distribution of monoamine oxidase A and B in rat brain was investigated using a coupled peroxidatic technique with benzylamine and tyramine as substrates and clorgyline and (−)-deprenyl as selective inhibitors. Benzylamine oxidase was absent in all areas. Both forms of monoamine oxidase were present, at low levels, in all areas; in addition several regions showed high activity of one or other form or both. Substantial activity of monoamine oxidase B was identified in the pineal gland, the lining of the ventricles, several hypothalamic regions, and the raphe nuclei. The locus coeruleus and interpeduncular nucleus possessed considerable type A activity. The substantia nigra and striatum showed no staining above the low general level, although the ventral tegmental area showed higher levels of both A and B. In general, noradrenaline-containing neuronal cell body areas showed monoamine oxidase A, and 5-hydroxytryptamine-rich areas monoamine oxidase B. There was no consistent enrichment of either in corresponding dopamine-rich regions. Monoamine oxidase thus appears to have a different role in these three types of neuron. The low level of monoamine oxidase B in the nigrostriatal tract may help to explain the resistance of the rat to MPTP toxicity.Keywords
This publication has 31 references indexed in Scilit:
- MPTPClinical Neuropharmacology, 1986
- Histochemistry of MPTP oxidation in the rat brain: Sites of synthesis of the parkinsonism-inducing toxin MPP+Neuroscience Letters, 1986
- III. Primate model of parkinsonism: Selective lesion of nigrostriatal neurons by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine produces an extrapyramidal syndrome in rhesus monkeysLife Sciences, 1985
- Monoamine oxidase-containing neurons in the cat hypothalamus: distribution and ascending projection to the cerebral cortexBrain Research, 1984
- Oxidation of the neurotoxic amine 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) by monoamine oxidases A and B and suicide inactivation of the enzymes by MPTPBiochemical and Biophysical Research Communications, 1984
- Amine oxidase activities in brown adipose tissue of the rat: identification of semicarbazide-sensitive (clorgyline-resistant) activity at the fat cell membraneJournal of Pharmacy and Pharmacology, 1984
- Histochemical investigation of criteria for the distinction between monoamine oxidase A and B in various species.Journal of Histochemistry & Cytochemistry, 1984
- Deamination of 5‐Hydroxytryptamine by Both Forms of Monoamine Oxidase in the Rat BrainJournal of Neurochemistry, 1982
- Do rat tissues possess 5‐hydroxytryptamine‐oxidizing activity distinct from monoamine oxidase A?Journal of Neurochemistry, 1979
- Histochemical characterization of monoamine oxidase in ependyma of rat hypothalamusJournal of Molecular Histology, 1979