A Comparative Analysis of theIn VitroEffects of Pulsed Electromagnetic Field Treatment on Osteogenic Differentiation of Two Different Mesenchymal Cell Lineages
Open Access
- 1 August 2013
- journal article
- research article
- Published by Mary Ann Liebert Inc in BioResearch Open Access
- Vol. 2 (4), 283-294
- https://doi.org/10.1089/biores.2013.0016
Abstract
Human mesenchymal stem cells (MSCs) are a promising candidate cell type for regenerative medicine and tissue engineering applications. Exposure of MSCs to physical stimuli favors early and rapid activation of the tissue repair process. In this study we investigated the in vitro effects of pulsed electromagnetic field (PEMF) treatment on the proliferation and osteogenic differentiation of bone marrow MSCs (BM-MSCs) and adipose-tissue MSCs (ASCs), to assess if both types of MSCs could be indifferently used in combination with PEMF exposure for bone tissue healing. We compared the cell viability, cell matrix distribution, and calcified matrix production in unstimulated and PEMF-stimulated (magnetic field: 2 mT, amplitude: 5 mV) mesenchymal cell lineages. After PEMF exposure, in comparison with ASCs, BM-MSCs showed an increase in cell proliferation (pppp<0.001) and its protein content was 1.2-fold higher in osteoblasts derived from BM-MSCs. The quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis revealed up-regulated transcription specific for bone sialoprotein, osteopontin, osteonectin, and Runx2, but at a higher level for cells differentiated from BM-MSCs. All together these results suggest that PEMF promotion of bone extracellular matrix deposition is more efficient in osteoblasts differentiated from BM-MSCs.Keywords
This publication has 68 references indexed in Scilit:
- Same or Not the Same? Comparison of Adipose Tissue-Derived Versus Bone Marrow-Derived Mesenchymal Stem and Stromal CellsStem Cells and Development, 2012
- Perfusion regulation of hMSC microenvironment and osteogenic differentiation in 3D scaffoldBiotechnology & Bioengineering, 2011
- Stem cells from adipose tissueCellular & Molecular Biology Letters, 2011
- Effects of 50-Hz magnetic field exposure on hormone secretion and apoptosis-related gene expression in human first trimester villous trophoblasts in vitroBioelectromagnetics, 2010
- Adipose-Derived Stem Cells: Characterization and Current Application in Orthopaedic Tissue RepairExperimental Biology and Medicine, 2009
- Adipose-derived stem cells: Isolation, expansion and differentiation☆Methods, 2008
- Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell‐therapy approaches: Further insights in the search for a fetal calf serum substituteJournal of Cellular Physiology, 2006
- Pulsed electromagnetic field treatments enhance the healing of fibular osteotomiesJournal of Orthopaedic Research, 2005
- In Vitro and In Vivo Effects of the Overexpression of Osteopontin on Osteoblast Differentiation Using a Recombinant Adenoviral VectorThe Journal of Biochemistry, 2004
- Regulation of alkaline phosphatase and alpha2(I) procollagen synthesis during early intramembranous bone formation in the rat mandibleDifferentiation, 1990