Cloning and expression of the hydrogenase gene fromClostridium butyricuminEscherichia coli
- 11 July 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 158 (1), 119-122
- https://doi.org/10.1016/0014-5793(83)80689-9
Abstract
Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd−) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03–0.04, C. butyricum and E. coli C600 (Hyd+).Keywords
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