Accurate transcription of cloned Neurospora RNA polymerase II-dependent genes in vitro by homologous soluble extracts.
- 1 August 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (16), 5450-5454
- https://doi.org/10.1073/pnas.82.16.5450
Abstract
We have developed soluble extracts from Neuropora crassa capable of accurately initiating the transcription of cloned Neurospora protein-encoding genes by RNA polymerase II in vitro. The genes encoding glutamate dehydrogenase (am) an histones H3 and H4 were transcribed by the extracts, and transcription was sensitive to .alpha.-amanitin at 1 mg/ml. The 5'' heterogeneity of the in vitro initiation reactions was highly specific. Of the 17 transcription initiation sites within the inducible qa gene cluster, only one minor site was used in vitro, suggesting that, in general, transcription from qa gene promoters requires at least one different protein from those required for transcription of the am and histone genes.This publication has 25 references indexed in Scilit:
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