Packaging recombinant DNA molecules into bacteriophage particles in vitro.
- 1 August 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (8), 3259-3263
- https://doi.org/10.1073/pnas.74.8.3259
Abstract
Recombinant phage [.lambda.] genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.This publication has 28 references indexed in Scilit:
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