Phenylalanyl-tRNA Synthetase from Baker’s Yeast: Specificity and Quantitation of Affinity Elution with tRNA
- 1 January 1976
- journal article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 357 (1), 819-824
- https://doi.org/10.1515/bchm2.1976.357.1.819
Abstract
TRNAPhe is able to elute phenylalanyl-tRNA synthetase from cation exchangers in a 1:1 ratio. Elution of phenylalanyl-tRNA synthetase in a 1:1 ratio is also observed for four noncognate tRNAs investigated, specific for valine, serine, isoleucine and tyrosine. If protein mixtures are subjected to affinity elution the cognate pair [tRNAPhe-phenylalanyl-tRNA synthetase] is eluted first, followed by noncognate pairs. The unspecific elution is not influenced by complexation of phenylalanyl-tRNA synthetase with an analog of phenylalanyl-adenylate.Keywords
This publication has 3 references indexed in Scilit:
- Phenylalanyl-tRNA synthetase from baker's yeast: role of 3'-terminal adenosine of tRNA-Phe in enzyme-substrate interaction studied with 3'-modified tRNA-Phe species.Proceedings of the National Academy of Sciences, 1975
- Synthese von Inhibitoren für die Phenylalanyl‐tRNA‐Synthetase: Methylen‐Analoge des Phenylalanyl‐adenylatsEuropean Journal of Inorganic Chemistry, 1973
- Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gelsBiochemical and Biophysical Research Communications, 1967