Current High Performance Liquid Chromatographic Methodology in Analysis of Nucleotides, Nucleosides, and Their Bases. II

Abstract

This review of HPLC technology is presented in two parts, the first of which appeared in Volume 1, Number 5 of this journal. Part I presented an introduction to chromatography, sample preparation, and basic information on chromatographic theory and terminology, modes, and instrumentation. Also covered in Part I was a discussion of stationary and mobile phase parameters which can be varied to optimize separations. Part II of the review which follows covers peak identification, quantification, and selected applications of HPLC for metabolic profiling, for assays of adenosine and cyclic nucleotides in studies of disease processes and for enzyme assays. The paper concludes with a short discussion of future trends for HPLC in biomedical research and clinical chemistry.