Incorporation of Double-Labelled Valine into δ-(L-α-Aminoadipyl)-L-Cysteinyl-D-Valine BYPenicillium chrysogenum

Abstract
The incorporation of valine into the LLD-tripeptide, δ-(L-α-amino-adipyl)-L-cysteinyl-D-valine, a precursor of penicillin, was studied by incubating mycelial mats of Penicillium chrysogenum Wis. 49–2105 with double labelled valines. L-valine was incorporated into the LLD-tripeptide without formation of an α, β-didehydrovaline intermediate. Intact D-valine was not incorporated into the LLD-tripeptide.