SEPARATION OF ARGINASE ISOENZYMES FROM HUMAN TISSUES BY AGAR-GEL ELECTROPHORESIS

Abstract

Arginase (EC 3.5.3.1) from human liver, kidney, mammary gland and erythrocytes, was separated by agar-gel electrophoresis using barbital buffer pH 8.6. Three isoenzymes were separated; 2 of these, A2 and A3, occur in liver and erythrocytes. The same 2 isoenzymes were found in the kidney, but in reversed proportions. In addition to the A3 isoenzyme, the mammary gland contains a fast anodically moving A1 isoenzyme. The 3 isoenzymes differ in their degree of sensitivity to ornithine.