Detection of Epstein‐Barr virus in Reed‐Sternberg cells of Hodgkin's disease arising in children
- 1 January 1993
- journal article
- research article
- Published by Wiley in Medical and Pediatric Oncology
- Vol. 21 (1), 19-23
- https://doi.org/10.1002/mpo.2950210105
Abstract
Nonisotopic in situ hybridization has been used to investigate the role of Epstein-Barr virus (EBV) in the aetiology of pediatric Hodgkin's disease. Sections from 24 cases arising in children under the age of 15 years were hybridised with digoxigenin-labelled probes for both EBV and cytomegalovirus, and reactive sites were identified by a sensitive three-layer immunoperoxidase technique. EBV was identified in Reed-Sternberg and mononuclear Hodgkin's cells in five samples (21%). No samples were positive when the cytomegalovirus probe was employed. The specific identification of EBV in the malignant cells of Hodgkin's disease arising in children lends further support for a role of EBV in the aetiology of this disorder.Keywords
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