The Structure of Teichoic Acid from Bacillus subtilis var. niger WM as Determined by 13C Nuclear‐Magnetic‐Resonance Spectroscopy
- 1 February 1976
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 62 (1), 1-6
- https://doi.org/10.1111/j.1432-1033.1976.tb10090.x
Abstract
The walls of Bacillus subtilis var. niger WM, grown in a Mg2+ -limited chemostat culture (carbon source glucose, dilution rate = 0.2 h(-1), 37 degrees C, pH 7) contained 45% (w/w) teichoic acid, a polymer composed of glycerol, phosphate[ and glucose in the molar ratio 1.00:1.00:0.88, respectively. Alkaline hydrolysis of this teichoic acid yeilded 1-O-beta-glucosylglycerol phosphate (together with small amounts of glycerol phosphate0 and 13C nuclear magnetic resonance spectra of this hydrolysis product, and its derivative after alkaline phosphate treatment, confirmed that the monomeric unit was 1-O-beta-glucosylglycerol-3-phosphate. Assignment of the resonances in the spectrum of undergraded teichoic acid revealed that the polymer was a poly [(2,3) glycerol phosphate 1, glucosidically substituted on C-1 of glycerol with beta-glucose.Keywords
This publication has 11 references indexed in Scilit:
- The Carbon-13 Nuclear Magnetic Resonance Spectra of 2-Acetamido-2-deoxy-D-hexoses and some Specifically Deuteriated, O-Acetylated, and Phosphorylated DerivativesCanadian Journal of Chemistry, 1973
- The anionic polymers in the cell wall of Bacillus subtilis var. niger grown in phosphorus-limiting environments supplemented with increasing concentrations of sodium chlorideBiochemical Journal, 1971
- The wall content and composition of Bacillus subtilis var. niger grown in a chemostatBiochemical Journal, 1970
- Control of teichoic acid and teichuronic acid biosynthesis in chemostat cultures of Bacillus subtilis var. nigerBiochemical Journal, 1969
- The glycerol teichoic acid from the cell wall of Bacillus stearothermophilus B65Biochemical Journal, 1966
- The Synthesis of Teichoic AcidsPublished by Elsevier ,1966
- The Role of Polyamines in the Neutralization of Bacteriophage Deoxyribonucleic AcidJournal of Biological Chemistry, 1960
- [12] Colorimetric analysis of sugarsMethods in Enzymology, 1957
- 551. Chemical studies in the biosynthesis of purine nucleotides. Part I. The preparation of N-glycylglycosylaminesJournal of the Chemical Society, 1956
- Detection of Sugars on Paper ChromatogramsNature, 1950