1H-NMR Assignments of the Non-exchangeable Protons of the Consensus Donor Exon:Intron Junction d(CpApGpGpTpApApGpT)
- 1 June 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 2 (6), 1125-1135
- https://doi.org/10.1080/07391102.1985.10507628
Abstract
The consensus donor exon:intron junction d(CpApGpGpTpApApGpT) has been synthesized by a modified phosphotriester method. The non-self-complementary nonamer has, in principle, only two G,C or four A,T points of self-recognition. The inference that it exists in the single strand form at 20°C was confirmed by temperature variable 1H-NMR and NOE measurements. The proton assignments were secured using two-dimensional COSY which provided intra-nucleotide correlations, then NOE difference measurements as well as inversion recovery T1 experiments. Systematic procedures were developed for the assignment of the individual bases and their component protons based on the effects of molecular environment on chemical shifts. These latter procedures should be useful for the assignment of other random-coil single strand oligodeoxyribonucleotides.This publication has 42 references indexed in Scilit:
- Two-dimensional proton nuclear magnetic resonance investigation of the synthetic deoxyribonucleic acid decamer d(ATATCGATAT)Biochemistry, 1983
- Assignment of the non-exchangeable proton resonances of d(C-G-C-G-A-A-T-T-C-G-C-G) using two-dimensional nuclear magnetic resonance methodsJournal of Molecular Biology, 1983
- Nuclear Overhauser effect as a tool for the complete assignment of nonexchangeable proton resonances in short deoxyribonucleic acid helixesBiochemistry, 1983
- Sequence-specific recognition of deoxyribonucleic acid. Chemical synthesis and nuclear magnetic resonance assignment of the imino protons of .lambda. OR3 operator deoxyribonucleic acidBiochemistry, 1983
- Proton nuclear Overhauser effect study of the structure of a deoxyoligonucleotide duplex in aqueous solutionBiochemistry, 1983
- Conformational study of two short pentadeoxyribonucleotides, CpCpApApG and CpTpTpGpG, and their fragments by proton NMRBiochemistry, 1982
- Premelting and melting transitions in the d(CGCGAATTCGCG) self-complementary duplex in solutionBiochemistry, 1982
- An immunoglobulin deletion mutant with implications for the heavy-chain switch and RNA splicingNature, 1980
- Splicing of yeast tRNA precursors: structure of the reaction intermediatesCell, 1979
- Nucleotide sequence homology at 12 intron–exon junctions in the chick ovalbumin geneNature, 1978