Simple Method for Analysis of Apolipoproteins in High Density Lipoproteins by High Performance Liquid Chromatography1

Abstract

A simple and rapid method has been developed for the separation of apolipoproteins in high density lipoprotein (HDL) fractions by high performance liquid chromatography (HPLQ with gel permeation columns (G3000SW TSK GEL). The HPLC pattern monitored by A₂₈₀ for a mixed solution of the HDL fraction (10 μl) and an eluent buffer (200 μl, 0.1 M sodium phosphate buffer containing 0.1% SDS) incubated at 60°C for 5 min showed two completely separated peaks which corresponded to the major components of human HDL, apolipoprotein A-I and A-II. Moreover, quantitation of apolipoprotein A-I by our method was found to correlate well with that by a single radial immunodiffusion (SRJD) assay.