Synthesis of a Cross-Linked Branched Polymer Network in the Interior of a Protein Cage
- 5 March 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 131 (12), 4346-4354
- https://doi.org/10.1021/ja8079862
Abstract
A goal of biomimetic chemistry is to use the hierarchical architecture inherent in biological systems to guide the synthesis of functional three-dimensional structures. Viruses and other highly symmetrical protein cage architectures provide defined scaffolds to initiate hierarchical structure assembly. Here we demonstrate that a cross-linked branched polymer can be initiated and synthesized within the interior cavity of a protein cage architecture. Creating this polymer network allows for the spatial control of pendant reactive sites and dramatically increases the stability of the cage architecture. This material was generated by the sequential coupling of multifunctional monomers using click chemistry to create a branched cross-linked polymer network. Analysis of polymer growth by mass spectrometry demonstrated that the polymer was initiated at the interior surface of the cage at genetically introduced cysteine reactive sites. The polymer grew as expected to generation 2.5 where it was limited by the size constraints of the cavity. The polymer network was fully cross-linked across protein subunits that make up the cage and extended the thermal stability for the cage to at least 120 °C. The introduced reactive centers were shown to be active and their number density increased with increasing generation. This synthetic approach provides a new avenue for creating defined polymer networks, spatially constrained by a biological template.Keywords
This publication has 46 references indexed in Scilit:
- Correct charge state assignment of native electrospray spectra of protein complexesJournal of the American Society for Mass Spectrometry, 2009
- Biomimetic synthesis of β-TiO2 inside a viral capsidJournal of Materials Chemistry, 2008
- Floating nanodot gate memory fabrication with biomineralized nanodot as charge storage nodeJournal of Applied Physics, 2008
- Viral capsids as templates for the production of monodisperse Prussian blue nanoparticlesChemical Communications, 2008
- Targeting of Cancer Cells with Ferrimagnetic Ferritin Cage NanoparticlesJournal of the American Chemical Society, 2006
- Structure of the DPS-Like Protein from Sulfolobus solfataricus Reveals a Bacterioferritin-Like Dimetal Binding Site within a DPS-Like Dodecameric Assembly,Biochemistry, 2006
- Paramagnetic viral nanoparticles as potential high‐relaxivity magnetic resonance contrast agentsMagnetic Resonance in Medicine, 2005
- Virus Crystals as Nanocomposite ScaffoldsJournal of the American Chemical Society, 2005
- Selective attachment and release of a chemotherapeutic agent from the interior of a protein cage architectureChemical Communications, 2004
- Natural Nanochemical Building Blocks: Icosahedral Virus Particles Organized by Attached OligonucleotidesNano Letters, 2004