Cloning and Expression of a Cocaine-Sensitive Dopamine Transporter Complementary DNA
- 25 October 1991
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 254 (5031), 576-578
- https://doi.org/10.1126/science.1948034
Abstract
A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and gamma-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT ([3H]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane) to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.Keywords
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