Construction of Human Gene Libraries from Small Amounts cf Peripheral Blood: Analysis of β-Like Globin Genes
- 31 December 1981
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 6 (1), 27-36
- https://doi.org/10.3109/03630268208996930
Abstract
A rapid procedure for constructing cloned human genomic libraries from small amounts of peripheral blood is described. High MW DNA is isolated from 5-20 ml peripheral blood, partially cleaved with Eco R1, and 8-22 kb [kilobase] fragments are cloned using bacteriophage Charon 4A and a suitable Escherichia coli host. Several non-.alpha. globin clones were isolated and characterized from a Kurdish Jew with homozygous .beta. thalassemia. The ability to isolate suitable amounts of high MW DNA from peripheral blood provides a relatively simple means of constructing human gene libraries representing a variety of Hb disorders.This publication has 19 references indexed in Scilit:
- Complete nucleotide sequence of the human δ-globin geneCell, 1980
- The nucleotide sequence of the human β-globin geneCell, 1980
- Human fetal gγ- and Aγ-globin genes: Complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genesCell, 1980
- The primary structure of the human ε-globin geneCell, 1980
- The isolation of structural genes from libraries of eucaryotic DNACell, 1978
- Identification of a Nondeletion Defect in α-ThalassemiaNew England Journal of Medicine, 1977
- Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA CloningScience, 1977
- A general method for isolation of high molecular weight DNA from eukaryotesNucleic Acids Research, 1976
- Agarose slab-gel electrophoresis equipmentAnalytical Biochemistry, 1975
- Isolation of High‐Molecular‐Weight DNA from Mammalian CellsEuropean Journal of Biochemistry, 1973