β-Adrenergic Stimulation of Adenosine-3’,5’-Monophosphate (c-AMP) Accumulation and of Prolactin and Growth Hormone Secretion in Rat Anterior Pituitary Cell Cultures
- 1 January 1985
- journal article
- research article
- Published by S. Karger AG in Neuroendocrinology
- Vol. 40 (1), 72-77
- https://doi.org/10.1159/000124054
Abstract
The .beta.-adrenergic agonist L-isoproterenol (ISO) (10-9-10-7 M) provoked a prompt and profound increase of intracellular cAMP accumulation in monolayer cultures of rat anterior pituitary. In superfused reaggregate cell cultures ISO also stimulated cAMP outflow. The effect was blocked by propranolol and the highly selective .beta.2-receptor blocker ICI 118.551 [erythro-dl-1-(7-methylindan-4-yloxy)-3-isopropylaminobutan-2-ol]. Epinephrine (E), norepinephrine (NE) and the highly selective .beta.2-agonist zinterol (ZIN) also stimulated cAMP accumulation. The order of potency ZIN .gtoreq. ISO > E .mchgt. NE together with the high potency of ICI 118.551 suggests the .beta.-effect is mainly through the .beta.2-receptor subtype. The same concentration of ISO strongly stimulated growth hormone (GH) release and, as previously shown, prolactin (PRL) release from superfused reaggregate cell cultures, but not luteinizing hormone or TSH release. Stimulation of PRL and GH release from these cultures was also induced by the adenylate cyclase activator forskolin. Some interference of .beta.-adrenergic effects on contaminating fibroblasts or endothelial cells could be reasonably excluded. .beta.-Adrenergic stimulation of cAMP accumulation in anterior pituitary cells apparently elicits GH and PRL release.This publication has 12 references indexed in Scilit:
- Characterization of β-adrenergic receptors in rat brain and pituitary using a new high-affinity ligand, [125I]iodocyanopindololBrain Research, 1983
- GLUCOCORTICOIDS INCREASE GTP-DEPENDENT ADENYLATE-CYCLASE ACTIVITY IN CULTURED FIBROBLASTS1983
- Physiological Concentrations of Dopamine Decrease Adenosine 3′,5′-Monophosphate Levels in Cultured Rat Anterior Pituitary Cells and Enriched Populations of Lactotrophs: Evidence for a Causal Relationship to Inhibition of Prolactin Release*Endocrinology, 1982
- β-ADRENERGIC STIMULATION OF PROLACTIN RELEASE FROM SUPERFUSED PITUITARY CELL AGGREGATES1Endocrinology, 1982
- Cerebral endothelial cell culture I. The presence of β2 and α2-adrenergic receptors linked to adenylate cyclase activityLife Sciences, 1982
- Evidence that ICI 118, 551 is a potent, highly beta2-selective adrenoceptor antagonist and can be used to characterize beta-adrenoceptor populations in tissuesLife Sciences, 1980
- PHARMACOLOGICAL SPECIFICITY OF BETA-1 AND BETA-2 ADRENERGIC-RECEPTORS IN RAT-HEART AND LUNG INVITRO1979
- Monolayer Cultures of Gonadotrophs Separated by Velocity Sedimentation: Heterogeneity in Response to Luteinizing Hormone-Releasing Hormone*Endocrinology, 1978
- ALPHA-ADRENERGIC RECEPTOR MODULATION OF BETA-ADRENERGIC, ADENOSINE AND PROSTAGLANDIN-E1 INCREASED ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE LEVELS IN PRIMARY CULTURES OF GLIA1978
- Differentiation of Receptor Systems activated by Sympathomimetic AminesNature, 1967