Autoradiographic tracing of developing subcortical projections of the occipital region in fetal rabbits

Abstract

Thirty rabbit embryos and two neonates (E18‐P1) received micropipette injections of ³H‐Leucine into the occipital region of one hemisphere and were killed after 0.5–5 hours. Incorporated tracer was demonstrated by autoradiography of serial sections of the brains. The first axons were seen in the intermediate zone of the developing cerebral cortex, on day E20, and by day E22 they reached the internal capsule. The entire cortico‐peduncular bundle and a short branch of the superficial (thalamic) bundle were labeled on day E24. On day E25, additional branches directed to claustrum, thalamus (deep bundle), and cerebellum were distinguished. By day E28 the first indications of terminal field development were observed. One day before birth (E30), the neonate pattern of subcortical pathways was fully established and silver grain condensations were present over most of the subcortical target areas. Subcortically, the axons followed preferentially preexisting fiber tracts. There was a period of at least 2–3 days between the arrival of the supplying bundles at the target sites and the onset of terminal field formation: The axon bundles grew first towards more distal targets, and even beyond, before terminal fields developed proximally. Transient axon bundles reaching the cerebellar paraflocculus and traveling along the pyramidal tract and the external and extreme capsules failed to form terminal fields and disappeared around birth. The data suggest that growth of long axonal tracts and the development of terminal fields are separate phenomena possibly regulated by different mechanisms.