Effect of pretreatment with hydroxylated xenobiotics on the activities of rat liver UDP-glucuronosyl-transferases

Abstract

1. Six hydroxylated substrates were examined as potential inducers of UDP-glucuronosyltransferases towards their conjugation and the conjugation of other ‘model’ aglycones with glucuronic acid. Time-dependence (4,14 and 28 days) and dose-dependence of treatments (from 25 mg/kg to 1 g/kg) were examined for some of these compounds. 2. Monoterpenoid alcohols (borneol and terpineol) did not enhance the glucuronidation of the ten substrates tested. p-Hydroxybiphenyl gives typical ‘substrate-induction’ towards its own conjugation. 3. Eugenol, a previously described inducer of UDP-glucuronosyltransferase activities in mouse, and 4-methylumbelliferone, give a general enhancement of all activities tested, especially those of the ‘3-methylcholanthrene-inducible’ group of aglycones. p-Nitrophenol at low dose (2.5 mg/kg) gives a limited ‘polycyclic aromatic hydrocarbon’-like increase.