The protein kinase inhibitor, K‐252a, decreases elicitor‐induced Ca2+ uptake and K+ release, and increases coumarin synthesis in parsley cells
- 11 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (1), 141-144
- https://doi.org/10.1016/0014-5793(91)80269-9
Abstract
An elicitor preparation from fungal cell walls known to induce coumarin synthesis in suspension-cultured parsley cells also elicits a rapid and transient Ca2+ uptake, K+ release and external alkalinization, and increases uptake of 45Ca2+ into the cells. The latter three responses were inhibited by the protein kinase inhibitor K-252a at 0.2 μM. Elicitor-induced coumarin synthesis, a process which requires gene activation, was greatly enhanced by K-252a. These results suggest that protein phosphorylation might be involved in the initial steps of signal transduction as well as in the long-term induction of coumarin synthesisKeywords
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