Sequence analysis of minute amounts of viroid RNA using the polymerase chain reaction (PCR)
- 1 September 1989
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 106 (3-4), 335-340
- https://doi.org/10.1007/bf01313962
Abstract
The amount of viroid RNA required for sequence analysis can be reduced by five to six orders of magnitude when a modified “polymerase chain reaction” (PCR) is used for the amplification of the reversely transcribed, overlapping viroid cDNAs. By applying this procedure it is possible to establish the molecular structure also of those viroids which are present only in extremely low amounts in various crop plants and ornamentals.This publication has 21 references indexed in Scilit:
- How to build an inexpensive Cyclotherm instrument for automated polymerase chain reactionAnalytical Biochemistry, 1989
- A microscale procedure for isolating and sequencing the viroid RNA present in one gram of infected leaf tissueJournal of Virological Methods, 1989
- A Definition of Citrus Viroid Groups and Their Relationship to the Exocortis DiseaseJournal of General Virology, 1988
- An improved procedure for the rapid one-step-cloning of full-length viroid cDNAArchiv für die gesamte Virusforschung, 1988
- Isolation of Three Viroids and a Circular RNA from GrapevinesJournal of General Virology, 1988
- Molecular and biological properties of a cloned and infectious new sequence variant of cucumber pale fruit virold (CPFV)Nucleic Acids Research, 1988
- A Viroid Resembling Hop Stunt Viroid in Grapevines from Europe, the United States and JapanJournal of General Virology, 1986
- Association of a viroid-like RNA from plum dapple disease occurring in Japan.Proceedings of the Japan Academy, Series B, 1986
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- A two-dimensional electrophoretic technique for the detection of circular viroids and virusoidsAnalytical Biochemistry, 1983