The Release of Nitrite from Barley Roots in Response to Metabolic Inhibitors, Uncoupling Agents, and Anoxia
- 1 February 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 30 (1), 119-133
- https://doi.org/10.1093/jxb/30.1.119
Abstract
When excised sterile barley roots, from plants which had been grown in the presence of nitrate, were placed under low oxygen tensions, nitrite was released into the external solution. The maximum leakage of nitrite occurred under completely anaerobic conditions. Nitrite was also released from barley roots under aerobic conditions when uncouplers of oxidative phosphorylation (DNP, CCCP1, pentachlorophenol) or certain simple organic acids were supplied. Inhibitors of the Krebs cycle, or of respiratory electron transport, were much less effective in causing the loss of nitrite, possibly because these compounds did not in general inhibit root respiration severely. Nitrite release, in response to any of the above treatments, was accompanied by an accumulation of nitrite within the tissue. It was concluded that an increase in membrane permeability, and a decrease in ATP synthesis, were contributory causes of this phenomenon, although neither could explain the experimental observations completely. There was however no evidence that the pentose phosphate pathway, which is regarded as the source of reducing power for nitrite reduction, was inhibited under conditions which favoured nitrite release.This publication has 17 references indexed in Scilit:
- [45] Nitrate reductase from higher plantsPublished by Elsevier ,1971
- Uncoupling of Energy-Linked Functions of Corn Mitochondria by Linoleic Acid and Monomethyldecenylsuccinic AcidPlant Physiology, 1967
- Loss of Organic Acids, Amino Acids, K, and Cl from Barley Roots Treated Anaerobically and with Metabolic InhibitorsPlant Physiology, 1967
- Respiratory Metabolism in Buckwheat SeedlingsPlant Physiology, 1967
- The Effect of Micro-organisms on the Absorption of Inorganic Nutrients by Intact Plants: I. APPARATUS AND CULTURE TECHNIQUEJournal of Experimental Botany, 1967
- The regulation of pathways of glucose catabolism in maize rootsBiochemical Journal, 1961
- Inhibition of Ion Absorption and Respiration in Barley Roots.Plant Physiology, 1955
- INHIBITION OF NITRATE ASSIMILATION IN EXCISED WHEAT ROOTS BY VARIOUS RESPIRATORY POISONSPlant Physiology, 1950
- NITRITE INJURY ON AVOCADO AND CITRUS SEEDLINGS IN NUTRIENT SOLUTION1Soil Science, 1949
- Studies on Fermentation in Rice and BarleyAmerican Journal of Botany, 1947