MURINE HYBRIDOMA‐DERIVED ANTIBODIES PRODUCING CIRCUMOVAL PRECIPITATION (COP) REACTIONS WITH EGGS OF SCHISTOSOMA JAPONICUM
- 1 August 1981
- journal article
- research article
- Published by Wiley in Immunology & Cell Biology
- Vol. 59 (4), 503-514
- https://doi.org/10.1038/icb.1981.43
Abstract
Of 7 hybridomas which secrete immunoglobulins binding to crude extracts of Schistosoma japonicum adult worms and/or eggs in solid-phase radioimmunoassays (RIAs), 3 gave positive precipitation reactions in the circumoval precipitin test (COPT). The COPT is a simple and inexpensive immunodiagnostic test for schistosomiasis japonica which involves the incubation of a selected batch of S. japonicum eggs with sera from patients and examination for precipitates one or more days later. Using a competitive RIA with an egg antigen extract and a labelled COPT-positive hybridoma ascites fluid, PwF.41-1-3, the surprising observation was made that only one anti-egg antibody specificity appeared to be represented in the series of 3 antibodies (as ascites fluids). Using sera as inhibitors in the competitive RIA, inhibitory activity (presumably antibodies to the target antigenic determinant of PwF.41-1-3) was readily detected in sera from egg-immunized mice and was of relatively high titre in a strain of mouse (C57BL/6) which can be readily sensitized for large granuloma formation around entrapped eggs in the lungs. Negligible inhibitory activity was found in the sera from S. japonicum-infected patients, even with sera from patients with prominent hepatosplenomegaly. The availability of COPT-positive hybridoma antibodies should facilitate isolation of at least one S. japonicum egg antigen involved in COP reactions and perhaps induction of immunopathological immune responses at least in mice.Keywords
This publication has 2 references indexed in Scilit:
- Partial Purification of Antigens from Eggs of Schistosoma Mansoni That Elicit Delayed HypersensitivityThe Journal of Immunology, 1977
- SCHISTOSOMA-MANSONI SOLUBLE EGG ANTIGENS .1. IDENTIFICATION AND PURIFICATION OF 3 MAJOR ANTIGENS, AND EMPLOYMENT OF RADIOIMMUNOASSAY FOR THEIR FURTHER CHARACTERIZATION1976