RELATIONSHIP BETWEEN TRINITROPHENYL AND H-2 ANTIGENS ON TRINITROPHENYL-MODIFIED SPLEEN-CELLS .2. CORRELATION BETWEEN DERIVATIZATION OF H-2 ANTIGENS WITH TRINITROPHENYL AND ABILITY OF TRINITROPHENYL-MODIFIED CELLS TO REACT FUNCTIONALLY IN CML ASSAY

Abstract

Spleen cells were modified with varying concentrations of trinitrobenzene sulfonic acid [TNBS] and then assayed for their ability to stimulate syngeneic spleen cells into displaying a cytotoxic effect against TNP[trinitrophenol]-modified target cells and for the extent of TNP derivatization of H-2 antigens. There was a direct correlation between the extent of derivatization of H-2 antigens and the ability of such derivatized cells to act as stimulator cells in the TNP-CML [cell-mediated lympholysis] assay. The altered self or interaction antigen hypothesis was supported as an explanation for the H-2 gene restriction of syngeneic CML. Target cells were also modified with TNBS at varying concentrations to determine the optimal concentration required to permit lysis in the CML assay. Similar concentration ranges of TNBS are required to create antigenic determinants on the target cells as well as immunogenic determinants on the stimulator cells that can be recognized by cytotoxic T [thymus-derived] cells.