Analysis of the DNAs from seven varicella-zoster virus isolates

Abstract

The 32P-labeled DNA from 7 different clinical isolates of human varicella-zoster virus (VZV) were independently digested with 5 site-specific restriction endonucleases, EcoRI, HindIII, SmaI, BamHI and AvaI. The digestion products were analyzed by electrophoresis on 0.5% agarose gels followed by autoradiography of the dried gels. Evaluation of the restriction enzyme cleavage patterns revealed small variations among the VZV DNA. The VZV DNA were also compared based on their buoyant densities in CsCl. No significant buoyant density differences were detected among the VZV DNA.