CDC25‐dependent induction of inositol 1,4,5‐trisphosphate and diacylglycerol in Saccharomyces cerevisiae by nitrogen
- 3 August 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 307 (3), 249-252
- https://doi.org/10.1016/0014-5793(92)80688-d
Abstract
The addition of ammonium sulfate to starved yeast cells leads to a 3- to 4-fold rapid increase of the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), the products of phosphoinositide-specific phospholipase C (PI-PLC). This response is reduced by dissecting the RAS-activating Cdc25 protein, and is completely abolished by the cdc25-1 mutation even at permissive temperature. Starved cdc25-1 mutant cells have a strongly reduced IP3 content, but an at least 10-fold increased DAG level compared to the isogenic wild-type strain. NH4 does not stimulate cAMP synthesis, and glucose does not induce IP3 and DAG. Our data suggest that the Cdc25 protein controls a nitrogen-specific signalling pathway involving the effector PI-PLC, in addition to the glucose-induced activation of adenylyl cyclase (AC).Keywords
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