Controlof Pseudomonas aeruginosa algZ Expression bythe Alternative Sigma FactorAlgT
- 15 December 2003
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (24), 7297-300
- https://doi.org/10.1128/jb.185.24.7297-7300.2003
Abstract
AlgZ controls Pseudomonas aeruginosa alginate synthesis by activating algD, yet algZ expression is not detectable in nonmucoid strains. Mobility shift and Western blot assays revealed that algZ expression requires the sigma factor AlgT. The mapped algZ transcription start site revealed a consensus AlgT-dependent promoter that, when mutated, substantially reduced algZ transcription.Keywords
This publication has 25 references indexed in Scilit:
- [14] Transcriptional analysis of genes involved in pseudomonas aeruginosa biofilmsMethods in Enzymology, 2001
- Integration-Proficient Pseudomonas aeruginosa Vectors for Isolation of Single-Copy Chromosomal lacZ and lux Gene FusionsBioTechniques, 2000
- Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogenNature, 2000
- Regulation of High Affinity Nickel Uptake in BacteriaJournal of Biological Chemistry, 2000
- Pseudomonas aeruginosa AlgZ, a ribbon–helix–helix DNA‐binding protein, is essential for alginate synthesis and algD transcriptional activationMolecular Microbiology, 1999
- Identification and characterization of AlgZ, an AlgT‐dependent DNA‐binding protein required for Pseudomonas aeruginosa algD transcriptionMolecular Microbiology, 1996
- The algT (algU) gene of Pseudomonas aeruginosa, a key regulator involved in alginate biosynthesis, encodes an alternative sigma factor (sigma E).Proceedings of the National Academy of Sciences, 1995
- An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosaGene, 1995
- DNA recognition by β-sheets in the Arc represser–operator crystal structureNature, 1994
- Pseudomonas aeruginosainfection in cystic fibrosis: nucleotide sequence and transcriptional regulation of thealgDgeneNucleic Acids Research, 1987