Increased Hemolytic Potency of Mouse Mammary Carcinoma Extracts Following Incubation with Tumor Cells.

Abstract

Filtered or centrifugated extracts prepd. from spontaneous mouse mammary carcinomas possess the ability to hemolyze mouse ery-throcytes in vitro. The present study shows that, if separated from the tumor cells by centrifugation, the mouse mammary carcinoma extracts lose their hemolytic potency at 37 C after 5 hrs. On the other hand, the hemolytic potency of the tumor extracts is either moderately or substantially increased following incubation in presence of tumor cells at 37 C for either 5 or 24 hrs. - Fresh extracts prepd. from normal mouse cells have in most instances no hemolytic potency, except for those made from active mouse mammary glands. If the normal mouse cell suspensions (liver, lungs, muscle, or pooled organs), however, are incubated at 37 C for 5 or, better, 24 hrs., some of them may show a slight hemolytic potency, though to a lesser degree than that observed in the case of pre-incubated tumor cell suspensions.