Two thymidylate synthetases in Bacillus subtilis.
Open Access
- 1 March 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (3), 1194-1198
- https://doi.org/10.1073/pnas.75.3.1194
Abstract
B. subtilis grown at temperatures below 37.degree. C contains 2 thymidylate synthetases, TSaseA and TSaseB. Their presence is dependent on functional thyA and thyB genes, respectively. When cells are grown at 46.degree. C they only contain TSaseA activity. This allows an easy positive selection for thyA and thyA,thyB mutants. The 2 TSases were physically separated, and they show similar overall requirements for activity. They differ significantly in both their kinetic and their physicochemical properties.This publication has 20 references indexed in Scilit:
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